1. Bacteriology and Antimicrobial Resistance

1. A cross-sectional phenotypic and genotypic analysis of ESBL-producing Escherichia coli isolates from urinary tract infections
2. A prospective cohort study of carbapenem-resistant Klebsiella pneumoniae colonization and subsequent infection rates in ICU patients
3. An in vitro time-kill assay comparison of novel β-lactam/β-lactamase inhibitor combinations against MDR Pseudomonas aeruginosa
4. A retrospective molecular epidemiology study of methicillin-resistant Staphylococcus aureus (MRSA) clones in hospital-acquired versus community-acquired infections
5. A randomized controlled laboratory evaluation of efflux pump inhibitors on tetracycline resistance reversal in Acinetobacter baumannii
6. A systematic review and meta-analysis of global prevalence trends of vancomycin-resistant Enterococcus species in clinical settings
7. An in vitro biofilm formation assay and antibiotic susceptibility profiling of clinical Staphylococcus epidermidis isolates from indwelling devices
8. A case-control study of plasmid-mediated colistin resistance gene (mcr-1) carriage in poultry versus human clinical isolates
9. A methodological validation of MALDI-TOF MS for rapid identification of non-fermenting Gram-negative bacilli in blood cultures
10. An in silico docking and molecular dynamics study of novel β-lactamase inhibitors against NDM-1 enzyme

2. Virology and Emerging Viral Diseases

1. A prospective observational study of dengue virus serotype distribution and viral load kinetics during an outbreak in a tertiary care hospital
2. An in vitro plaque reduction neutralization test for assessing the efficacy of candidate antiviral compounds against chikungunya virus
3. A cross-sectional molecular detection and phylogenetic analysis of SARS-CoV-2 variants in asymptomatic versus symptomatic patients
4. A retrospective cohort study of HBV viral load fluctuations and ALT levels in chronic hepatitis B patients undergoing antiviral therapy
5. An in vitro evaluation of viral entry inhibition by heparin derivatives against herpes simplex virus type-1 in Vero cells
6. A systematic review of zoonotic spillover risk factors for emerging bat-borne viruses in South Asia
7. An in vitro assessment of RNAi-mediated silencing of influenza A virus nucleoprotein in MDCK cell culture
8. A case-control seroepidemiological study of Crimean-Congo hemorrhagic fever virus exposure in livestock handlers
9. A methodological validation of loop-mediated isothermal amplification (LAMP) assay for rapid detection of rotavirus in pediatric stools
10. An in silico epitope mapping study for design of a pan-coronavirus vaccine candidate

3. Parasitology and Vector-Borne Diseases

1. A cross-sectional microscopy and PCR-based prevalence study of Plasmodium falciparum and P. vivax co-infections in endemic regions
2. A prospective trial comparing efficacy of artemisinin combination therapies versus monotherapy in uncomplicated malaria treatment outcomes
3. An in vitro axenic culture study of Leishmania donovani promastigote susceptibility to novel antileishmanial compounds
4. A case-control investigation of serological markers (antibody titres) in asymptomatic versus symptomatic visceral leishmaniasis cases
5. A community-based entomological survey of insecticide resistance mechanisms in Anopheles mosquitoes using WHO bioassays and PCR
6. A systematic review and meta-analysis of global prevalence of Toxoplasma gondii infection in pregnant women and congenital transmission rates
7. An in vitro evaluation of anti-parasitic activity of plant-derived alkaloids against Giardia lamblia trophozoites
8. A retrospective cohort study of clinical outcomes and immune response markers in neurocysticercosis patients treated with albendazole
9. A methodological study of loop-mediated isothermal amplification (LAMP) versus conventional PCR for detection of Trypanosoma cruzi DNA
10. An in silico population genetics analysis of Wuchereria bancrofti strains and their drug resistance-associated SNPs

4. Mycology and Antifungal Strategies

1. A cross-sectional laboratory evaluation of Candida species distribution and antifungal susceptibility patterns in bloodstream infections
2. A prospective in vivo murine model study of echinocandin efficacy against invasive candidiasis
3. An in vitro biofilm inhibition assay of novel synthesis-based small molecules against Candida auris
4. A retrospective analysis of galactomannan and β-D-glucan diagnostic performance in invasive aspergillosis suspicion cases
5. A randomized controlled laboratory trial comparing voriconazole and isavuconazole MIC distributions against clinical Aspergillus fumigatus isolates
6. A systematic review of global trends in antifungal resistance among dermatophytes and non-dermatophyte molds
7. An in vitro synergy study of amphotericin B and flucytosine combination therapy on Cryptococcus neoformans isolates
8. A case-control epidemiological study of fungal colonization rates in immunocompromised versus immunocompetent ICU patients
9. A methodological validation of MALDI-TOF MS database entries for rapid identification of emerging pathogenic yeasts
10. An in silico docking study of natural compounds as inhibitors of Candida albicans efflux pumps

5. Environmental and Industrial Microbiology

1. A comparative cross-sectional survey of bacterial community structure in municipal drinking water versus hospital potable water systems using 16S rRNA sequencing
2. A prospective experimental study on bioaugmentation efficacy for petroleum hydrocarbon degradation in contaminated soil microcosms
3. An in vitro assessment of microbial consortia for bioleaching of copper from low-grade ores under varying pH conditions
4. A case-control analysis of Legionella colonization in health-care facility water systems pre- and post-implementation of disinfectant protocols
5. A systematic review of industrial-scale microbial production of bioethanol: strain optimization and bioprocess parameters
6. An in vitro evaluation of quorum-quenching enzymes to control biofilm formation in food processing pipelines
7. A retrospective study of microbial quality indicators in bottled water brands and correlation with storage conditions
8. A methodological validation of biosensor-based detection of E. coli in recreational water bodies
9. An in vitro comparative study of probiotic viability in novel microencapsulation formulations for dairy applications
10. An in silico metabolic network reconstruction of Clostridium acetobutylicum for optimized butanol production

6. Immunology and Host–Pathogen Interactions

1. A prospective observational study of cytokine profiles (IL-6, TNF-α, IFN-γ) in acute leptospirosis versus dengue fever patients
2. An in vitro co-culture assay of macrophage polarization responses to Mycobacterium tuberculosis infection
3. A cross-sectional flow cytometry–based analysis of T-cell subset distributions in HIV-infected individuals with opportunistic infections
4. A randomized controlled trial of probiotic supplementation effects on gut mucosal immunity markers in antibiotic-treated patients
5. A systematic review and meta-analysis of vaccine-induced versus natural immunity antibody kinetics in varicella-zoster virus
6. An in vitro neutralization assay of monoclonal antibodies targeting RSV F protein in cell culture
7. A case-control evaluation of host genetic polymorphisms (TLR2, TLR4) and susceptibility to invasive pneumococcal disease
8. A retrospective histopathological and immunohistochemical study of granuloma architecture in tubercular versus sarcoid lymphadenitis
9. A methodological validation of ELISPOT assay for quantifying antigen-specific T-cell responses in vaccine trials
10. An in silico prediction of host–pathogen protein–protein interaction networks for Zika virus infection

7. Diagnostic Microbiology and Rapid Detection Methods

1. A cross-sectional validation of multiplex PCR panels versus culture methods for detection of respiratory pathogens in pneumonia patients
2. A prospective laboratory evaluation of CRISPR-Cas-based diagnostics for point-of-care detection of Clostridioides difficile
3. An in vitro analytical sensitivity and specificity assessment of lateral flow assays for rapid dengue NS1 antigen detection
4. A retrospective comparative study of automated blood culture systems versus manual methods for time-to-detection of bacteremia
5. A methodological validation of digital droplet PCR for quantification of Mycobacterium tuberculosis DNA in sputum samples
6. A systematic review of point-of-care molecular diagnostics for sexually transmitted infections: performance and feasibility
7. An in vitro study of phage-based biosensors for rapid identification of carbapenemase-producing Enterobacteriaceae
8. A case-control evaluation of acoustic biosensor technology for real-time detection of bacterial contamination in peritoneal dialysis fluids
9. A pilot experimental study of smartphone-integrated microscopy for detection of malarial parasites in resource-limited settings
10. An in silico meta-analysis of sensitivity and specificity data of novel nucleic acid amplification tests for TB diagnosis

8. Microbial Genetics and Genomics

1. A cross-sectional genomic characterization of persistent Staphylococcus aureus strains using whole-genome sequencing and SNP analysis
2. An in vitro CRISPR-Cas9 gene knockout study to elucidate essential genes for biofilm formation in Pseudomonas aeruginosa
3. A retrospective comparative genomics study of toxigenic versus non-toxigenic Vibrio cholerae isolates from outbreak investigations
4. A prospective transcriptomic profiling of antibiotic stress response in E. coli using RNA-Seq technology
5. A systematic review of pan-genome analysis methodologies and applications in pathogen diversity studies
6. An in vitro plasmid curing assay and stability testing in clinical Klebsiella pneumoniae isolates
7. A case-control investigation of mobile genetic element–mediated antimicrobial resistance gene transfer in hospital effluents
8. A methodological study on nanopore sequencing accuracy for detection of large structural variants in bacterial genomes
9. An in silico comparative analysis of CRISPR arrays across Mycobacterium species and their spacer diversity
10. A pilot experimental evolution study of antibiotic adaptation in microbial chemostat cultures monitored by whole-genome sequencing

9. Microbial Ecology and Bioremediation

1. A cross-sectional metagenomic analysis of soil microbial communities in pesticide-contaminated versus organic farms
2. A prospective microcosm study of microbial-mediated degradation of plastic polymers under simulated landfill conditions
3. An in vitro co-culture experiment of hydrocarbon-degrading consortia for enhancement of oil spill bioremediation
4. A case-control survey of gastrointestinal microbiota alterations in residents near industrial effluent sites versus controls
5. A systematic review of microbial electrochemical technologies for wastewater treatment and energy recovery
6. An in vitro assessment of heavy metal biosorption capacities of fungal biosorbents using isotherm models
7. A retrospective analysis of biogas-producing microbial community dynamics in large-scale anaerobic digesters
8. A methodological validation of stable isotope probing for tracking microbial metabolic activity in environmental samples
9. An in silico metabolic pathway reconstruction of petroleum-degrading bacteria using genome-scale models
10. A pilot field study of phytoremediation-microbe synergy for removal of chromium contamination from soil

10. Recent Advances in Microbiology

1. A scoping review of CRISPR-based antimicrobials: mechanisms, effectiveness, and delivery challenges
2. An in vitro validation of bacteriophage-derived endolysins against MDR bacterial biofilms
3. A prospective observational study of nanopore sequencing application for real-time outbreak investigation in hospital settings
4. A methodological assessment of AI-based image analysis for microbial colony morphology classification
5. An in silico network pharmacology study of microbiome-derived metabolites influencing human immune pathways
6. A pilot trial of synbiotic therapy effects on gut microbial metabolome in patients with inflammatory bowel disease
7. A systematic review of microfluidic lab-on-a-chip platforms for rapid pathogen detection in clinical samples
8. An experimental study of quorum-sensing inhibitors isolated from marine microorganisms against Vibrio spp.
9. A retrospective data-mining analysis of global antimicrobial peptide databases for novel therapeutic candidates
10. A narrative review of single-cell genomics technologies and their applications in microbiome research