1. Enzymology and Enzyme Kinetics

1. An in vitro experimental study of Michaelis–Menten kinetics of alkaline phosphatase in human serum under varying pH and ionic strength conditions
2. A comparative cross-sectional analysis of acetylcholinesterase inhibition by organophosphate pesticides in agricultural workers versus controls
3. A prospective observational study of lactate dehydrogenase isoenzyme profiles in patients with acute myocardial infarction
4. An in vitro temperature-dependent activity assay of erythrocyte catalase: a controlled laboratory investigation
5. A case-control study of glucose-6-phosphate dehydrogenase activity in G6PD-deficient versus non-deficient pediatric subjects
6. An in vitro substrate saturation study of pancreatic lipase in serum samples from chronic pancreatitis patients
7. A randomized comparative assay of carbonic anhydrase inhibition by sulfonamide derivatives: enzyme kinetics approach
8. A retrospective kinetic modeling of hexokinase activity in stored versus fresh blood samples: implications for glucose assay stability
9. An in vitro mutagenesis study on the effect of point mutations in tyrosinase catalytic efficiency
10. A systematic review and meta-analysis of enzyme kinetic alterations in inherited metabolic disorders

2. Metabolic Pathways and Flux Analysis

1. A stable-isotope tracer experimental study of gluconeogenesis flux in fasting versus fed states in healthy volunteers
2. A cross-sectional comparative profiling of tricarboxylic acid cycle intermediates in type 2 diabetic versus non-diabetic subjects
3. An in vitro flux analysis of pentose phosphate pathway activity in cultured hepatocytes exposed to oxidative stress
4. A prospective cohort study of serum homocysteine and methylation cycle metabolites in women with preeclampsia
5. A case-control investigation of branched-chain amino acid catabolism markers in patients with non-alcoholic fatty liver disease
6. An in vivo ^13C-NMR tracer study of hepatic glycogen synthesis rates in alcoholic versus non-alcoholic individuals
7. A comparative enzymatic assay study of fatty acid β-oxidation rates in mitochondrial extracts from obese and lean subjects
8. A retrospective analysis of metabolic fingerprinting data in septic shock patients using GC-MS
9. An in silico kinetic modeling study of glycolytic pathway regulation under hypoxic conditions
10. A systematic review of metabolic flux alterations in cancer cell lines: implications for targeted therapy

3. Clinical Biochemistry and Biomarkers

1. A prospective cross-sectional study of serum troponin I and CK-MB kinetics in early diagnosis of acute coronary syndrome
2. A case-control evaluation of serum C-reactive protein versus pentraxin-3 levels in rheumatoid arthritis activity assessment
3. A longitudinal observational study of HbA1c variability and microvascular complications in type 1 diabetes mellitus
4. A comparative diagnostic accuracy study of serum procalcitonin and CRP for neonatal sepsis in a tertiary care hospital
5. A randomized controlled trial assessing the effect of vitamin D supplementation on serum osteocalcin as a bone turnover marker
6. A cross-sectional profiling of urinary albumin-creatinine ratio and NGAL in early detection of diabetic nephropathy
7. A retrospective cohort study of serum ferritin as a prognostic biomarker in COVID-19 pneumonia
8. An in vitro ELISA validation study for novel cardiac biomarker copeptin in suspected heart failure patients
9. A nested case-control study of plasma miRNA-21 expression as a biomarker in breast cancer screening
10. A systematic review and meta-analysis of circulating exosomal miRNAs as diagnostic markers in neurological disorders

4. Molecular Biology Techniques in Biochemistry

1. An in vitro validation study of qRT-PCR primers for quantification of PPAR-γ mRNA in adipocyte differentiation
2. A comparative evaluation of Western blot versus ELISA for detection of phosphorylated Akt in insulin-stimulated cells
3. A methodological study on CRISPR/Cas9-mediated knockdown of GLUT4 in cultured skeletal myocytes
4. An in vitro optimization of protein purification protocols using His-tag affinity chromatography for liver enzymes
5. A cross-sectional laboratory assessment of next-generation sequencing accuracy on mitochondrial DNA heteroplasmy detection
6. An in vitro reporter assay study for NF-κB transcriptional activity under pro-inflammatory cytokine stimulation
7. A pilot experimental study of mass spectrometry–based proteomic profiling in cerebrospinal fluid samples
8. A technical validation of surface plasmon resonance for measuring enzyme-inhibitor interactions in real time
9. A methodological appraisal of flow cytometry–based reactive oxygen species detection in lymphocytes
10. A systematic review of molecular biology assay reproducibility in clinical biochemistry research

5. Nutritional Biochemistry

1. A randomized controlled dietary intervention study of high-protein versus high-carbohydrate diets on serum insulin and IGF-1 levels
2. A cross-sectional comparative analysis of serum vitamin B12 and methylmalonic acid in vegetarian versus non-vegetarian populations
3. A prospective cohort study of omega-3 fatty acid supplementation and changes in lipid peroxidation markers in elderly subjects
4. An in vitro assessment of quercetin’s effect on glucose uptake in cultured adipocytes: mechanistic insights
5. A case-control study of trace element (zinc, copper) status in children with protein-energy malnutrition
6. A randomized open-label trial of probiotic supplementation on gut permeability markers in irritable bowel syndrome
7. A cross-sectional survey of dietary antioxidants and serum total antioxidant capacity in urban versus rural adults
8. An in vitro digestion model study of bioavailability of curcumin nanoparticles under simulated gastrointestinal conditions
9. A retrospective analysis of nutritional biomarkers in critically ill patients: correlation with clinical outcomes
10. A systematic review of nutrigenomic interactions in the prevention of metabolic syndrome

6. Oxidative Stress, Free Radicals & Antioxidants

1. An in vitro cell culture study of reactive oxygen species generation by amyloid-β in neuronal cell lines
2. A comparative in vivo study of lipid peroxidation markers in smokers versus non-smokers using TBARS assay
3. A prospective observational study of serum 8-hydroxy-2′-deoxyguanosine levels in patients with chronic kidney disease
4. An in vitro antioxidant capacity assay of phytochemical extracts from traditional Indian medicinal plants
5. A randomized controlled trial of N-acetylcysteine supplementation on oxidative stress markers in COPD patients
6. A cross-sectional analysis of redox status (GSH/GSSG ratio) in type 2 diabetic versus healthy individuals
7. An in vitro mechanistic study of superoxide dismutase mimetics in endothelial cell protection
8. A retrospective cohort evaluation of oxidative DNA damage markers and cancer risk in a hospital registry
9. An in silico docking study of novel antioxidant compounds against NADPH oxidase active site
10. A systematic review of clinical trials on antioxidant therapy in neurodegenerative diseases

7. Genetic Biochemistry and Enzymopathies

1. A case-control genotyping study of G6PD variants and enzyme activity correlation in pediatric hemolytic anemia
2. An in vitro site-directed mutagenesis study on phenylalanine hydroxylase variants causing phenylketonuria
3. A prospective observational analysis of sphingomyelinase activity in Niemann–Pick disease carriers versus patients
4. An in silico modeling and validation of α-galactosidase A mutations in Fabry disease using homology modeling
5. A cross-sectional study of pyruvate kinase deficiency mutations and corresponding RBC metabolic profiles
6. A nested case-control evaluation of UGT1A1 promoter polymorphisms and serum bilirubin in neonates
7. An in vitro enzyme replacement study assessing recombinant lysosomal acid lipase uptake in cultured fibroblasts
8. A retrospective mutation spectrum analysis of hereditary hemochromatosis genes in an Indian cohort
9. An in vivo animal model study of gene therapy efficacy for succinate dehydrogenase deficiency
10. A systematic review of genotype–phenotype correlations in inherited metabolic enzymopathies

8. Signal Transduction and Cell Communication

1. An in vitro Western blot study of MAPK pathway activation in endothelial cells exposed to high glucose
2. A comparative cell-based assay of insulin receptor phosphorylation in adipocytes from obese versus lean donors
3. A prospective experimental investigation of JAK-STAT signaling modulation by curcumin in immune cells
4. An in vitro reporter gene assay for NF-κB activation by lipopolysaccharide in macrophage cultures
5. A randomized controlled trial of PI3K/Akt pathway inhibitors on tumor markers in breast cancer patients
6. A cross-sectional ELISA-based profiling of Wnt signaling modulators in colon carcinoma tissue samples
7. An in vitro co-culture study of Notch signaling effects on osteoblast differentiation
8. A retrospective phosphoproteomics analysis of cell signaling alterations in Alzheimer’s disease brain samples
9. An in silico pathway enrichment analysis of proteomic data from cytokine-stimulated hepatocytes
10. A systematic review of clinical interventions targeting signal transduction pathways in metabolic disorders

9. Bioinformatics, Proteomics and Metabolomics

1. An in silico comparative proteomic analysis of serum protein changes in dengue fever using publicly available datasets
2. An experimental LC-MS/MS–based metabolomic profiling study in urine samples of kidney stone patients
3. A retrospective data-mining study of gene expression microarray datasets in thyroid carcinoma
4. An in vitro validation of candidate biomarkers identified by shotgun proteomics in osteosarcoma cell lines
5. A cross-sectional bioinformatics pipeline evaluation for peptide identification in complex plasma samples
6. An in silico network pharmacology study of herbal compounds targeting diabetic nephropathy pathways
7. An experimental stable isotope labeling (SILAC) proteomics study of insulin resistance in hepatocyte models
8. A retrospective integrative omics analysis of transcriptomic and metabolomic data in sepsis patients
9. A methodological study comparing targeted versus untargeted metabolomics platforms for biomarker discovery
10. A systematic review of bioinformatics tools for multi-omics data integration in clinical research

10. Recent Studies in Biochemistry

1. A scoping review of CRISPR-based diagnostic assays developed for point-of-care detection of infectious diseases
2. An in vitro evaluation of SARS-CoV-2 main protease inhibitors identified by high-throughput screening
3. A prospective pilot study of exosome-based liquid biopsy biomarkers in early cancer detection
4. An experimental investigation of mRNA vaccine stability under varied storage conditions
5. An in silico drug repurposing study targeting metabolic enzymes in neurodegenerative disease
6. A cross-sectional multi-omics analysis of gut microbiome metabolites in inflammatory bowel disease
7. A retrospective review of nanoparticle-mediated enzyme delivery systems in clinical trials
8. An in vitro assessment of artificial intelligence–guided enzyme engineering for improved catalytic efficiency
9. A meta-analysis of fluorogenic probe development for real-time imaging of live-cell enzymatic activity
10. A narrative review of advances in single-cell metabolomics technologies and their biomedical applications